It's up to you to decide whether to use и or not. In colloquial speech, the rules aren't strict. But in written speech — especially in scientific works — they do exist.
If the last word in enumeration is added without the conjunction и, the chain is meant and perceived as unfinished and open for possible addition (in some cases, the phrases и так далее, и тому подобное, и прочее can be used).
An example from a scientific text:
Инфекции могут подразделяться на антропонозы (полиомиелит, менинго- кокковая инфекция, вирусный гепатит и др.) и зоонозы (бешенство, бруцеллез, лептоспироз, туляремия и пр.).
Enumeration is considered finished when the last word is linked with the conjunction и. The conjunction shows that the list is complete, exhaustive, and doesn't require additional information.
Музыкальные звуки различаются по силе, высоте и тембру.